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lambda fix ii vector  (Agilent technologies)


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    Structured Review

    Agilent technologies lambda fix ii vector
    Lambda Fix Ii Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+fix+ii/pmc00538280-103-14-18
    Average 90 stars, based on 1 article reviews
    lambda fix ii vector - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Recombinant:

    Article Title: Methods of gene therapy using nucleic acid sequences for ATP-binding cassette transporter
    Article Snippet: .. Insertional inactivation of the alpha-peptide allows recombinant clones to be identified directly by color screening on indicator plates. cDNA clones from various regions of the ABCR gene were used as probes to screen a human genomic library in Lambda FIX II (#946203, Stratagene, LaJolla, Calif.). ..

    Article Title: PUFA polyketide synthase systems and uses thereof
    Article Snippet: .. Finally, a recombinant genomic library, consisting of DNA fragments from Thraustochytrium 23B genomic DNA inserted into vector lambda FIX II (Stratagene), was screened using digoxigenin labeled probes corresponding to the following segments of Schizochytrium 20888 PUFA-PKS genes: nucleotides 7385-7879 of Orf A (SEQ ID NO:1), nucleotides 5012-5511 of Orf B (SEQ ID NO:3), and nucleotides 76-549 of Orf C (SEQ ID NO:5). ..

    Article Title: PUFA polyketide synthase systems and uses thereof
    Article Snippet: .. Finally, a recombinant genomic library, consisting of DNA fragments from Thraustochytrium 23B genomic DNA inserted into vector lambda FIX II (Stratagene), was screened using digoxigenin labeled probes corresponding to the following segments of Schizochytrium 20888 PUFA-PKS genes: nucleotides 7385-7879 of Orf A (SEQ ID NO:1), nucleotides 5012-5511 of Orf B (SEQ ID NO:3), and nucleotides 76-549 of Orf C (SEQ ID NO:5). ..

    Clone Assay:

    Article Title: Methods of gene therapy using nucleic acid sequences for ATP-binding cassette transporter
    Article Snippet: .. Insertional inactivation of the alpha-peptide allows recombinant clones to be identified directly by color screening on indicator plates. cDNA clones from various regions of the ABCR gene were used as probes to screen a human genomic library in Lambda FIX II (#946203, Stratagene, LaJolla, Calif.). ..

    Article Title: Transgenic non-human animals for producing chimeric antibodies
    Article Snippet: .. The 16 kb SmaI fraction is cloned into Lambda FIX II (Stratagene, La Jolla, Calif.) which has been digested with XhoI, treated with klenow fragment DNA polymerase to fill in the XhoI restriction digest product. ..

    Article Title: PVAS3, a class-II ubiquitous asparagine synthetase from the common bean (Phaseolus vulgaris).
    Article Snippet: A gene encoding a putative asparagine synthetase (AS; EC 6.3.5.4) has been isolated from common bean (Phaseolus vulgaris).. A 2.4 kb cDNA clone of this gene (PVAS3) encodes a protein of 570 amino acids with a predicted molecular mass of 64,678 Da, an isoelectric point of 6.45, and a net charge of -5.9 at pH 7.0.. The PVAS3 protein sequence conserves all the amino acid residues that are essential for glutamine-dependent AS, and PVAS3 complemented an E. coli asparagine auxotroph, that demonstrates that it encodes a glutamine-dependent AS.

    Article Title: Regulatory sequences for regulation of gene expression in plants and other organisms, and compositions, products and methods related thereto
    Article Snippet: .. Results About 200,000 clones from an oil palm genomic library constructed in lambda FIX II (Stratagene) were screened using random prime-labeled PCR produced probe containing pOPSN6 sequence. ..

    Construct:

    Article Title: Regulatory divergence of homeologous Atlantic salmon elovl5 genes following the salmonid-specific whole-genome duplication.
    Article Snippet: .. An Atlantic salmon genomic DNA library was constructed in lambda FIX II (Stratagene, USA, Zheng et al., 2009). ..

    Article Title: PVAS3, a class-II ubiquitous asparagine synthetase from the common bean (Phaseolus vulgaris).
    Article Snippet: A gene encoding a putative asparagine synthetase (AS; EC 6.3.5.4) has been isolated from common bean (Phaseolus vulgaris).. A 2.4 kb cDNA clone of this gene (PVAS3) encodes a protein of 570 amino acids with a predicted molecular mass of 64,678 Da, an isoelectric point of 6.45, and a net charge of -5.9 at pH 7.0.. The PVAS3 protein sequence conserves all the amino acid residues that are essential for glutamine-dependent AS, and PVAS3 complemented an E. coli asparagine auxotroph, that demonstrates that it encodes a glutamine-dependent AS.

    Article Title: Regulatory sequences for regulation of gene expression in plants and other organisms, and compositions, products and methods related thereto
    Article Snippet: .. Results About 200,000 clones from an oil palm genomic library constructed in lambda FIX II (Stratagene) were screened using random prime-labeled PCR produced probe containing pOPSN6 sequence. ..

    Plasmid Preparation:

    Article Title: PUFA polyketide synthase systems and uses thereof
    Article Snippet: .. Finally, a recombinant genomic library, consisting of DNA fragments from Thraustochytrium 23B genomic DNA inserted into vector lambda FIX II (Stratagene), was screened using digoxigenin labeled probes corresponding to the following segments of Schizochytrium 20888 PUFA-PKS genes: nucleotides 7385-7879 of Orf A (SEQ ID NO:1), nucleotides 5012-5511 of Orf B (SEQ ID NO:3), and nucleotides 76-549 of Orf C (SEQ ID NO:5). ..

    Labeling:

    Article Title: PUFA polyketide synthase systems and uses thereof
    Article Snippet: .. Finally, a recombinant genomic library, consisting of DNA fragments from Thraustochytrium 23B genomic DNA inserted into vector lambda FIX II (Stratagene), was screened using digoxigenin labeled probes corresponding to the following segments of Schizochytrium 20888 PUFA-PKS genes: nucleotides 7385-7879 of Orf A (SEQ ID NO:1), nucleotides 5012-5511 of Orf B (SEQ ID NO:3), and nucleotides 76-549 of Orf C (SEQ ID NO:5). ..

    Article Title: PUFA polyketide synthase systems and uses thereof
    Article Snippet: .. Finally, a recombinant genomic library, consisting of DNA fragments from Thraustochytrium 23B genomic DNA inserted into vector lambda FIX II (Stratagene), was screened using digoxigenin labeled probes corresponding to the following segments of Schizochytrium 20888 PUFA-PKS genes: nucleotides 7385-7879 of Orf A (SEQ ID NO:1), nucleotides 5012-5511 of Orf B (SEQ ID NO:3), and nucleotides 76-549 of Orf C (SEQ ID NO:5). ..

    Isolation:

    Article Title: PVAS3, a class-II ubiquitous asparagine synthetase from the common bean (Phaseolus vulgaris).
    Article Snippet: A gene encoding a putative asparagine synthetase (AS; EC 6.3.5.4) has been isolated from common bean (Phaseolus vulgaris).. A 2.4 kb cDNA clone of this gene (PVAS3) encodes a protein of 570 amino acids with a predicted molecular mass of 64,678 Da, an isoelectric point of 6.45, and a net charge of -5.9 at pH 7.0.. The PVAS3 protein sequence conserves all the amino acid residues that are essential for glutamine-dependent AS, and PVAS3 complemented an E. coli asparagine auxotroph, that demonstrates that it encodes a glutamine-dependent AS.

    Polymerase Chain Reaction:

    Article Title: Regulatory sequences for regulation of gene expression in plants and other organisms, and compositions, products and methods related thereto
    Article Snippet: .. Results About 200,000 clones from an oil palm genomic library constructed in lambda FIX II (Stratagene) were screened using random prime-labeled PCR produced probe containing pOPSN6 sequence. ..

    Produced:

    Article Title: Regulatory sequences for regulation of gene expression in plants and other organisms, and compositions, products and methods related thereto
    Article Snippet: .. Results About 200,000 clones from an oil palm genomic library constructed in lambda FIX II (Stratagene) were screened using random prime-labeled PCR produced probe containing pOPSN6 sequence. ..

    Sequencing:

    Article Title: Regulatory sequences for regulation of gene expression in plants and other organisms, and compositions, products and methods related thereto
    Article Snippet: .. Results About 200,000 clones from an oil palm genomic library constructed in lambda FIX II (Stratagene) were screened using random prime-labeled PCR produced probe containing pOPSN6 sequence. ..

    other:

    Article Title: Carotene synthase gene and uses therefor
    Article Snippet: Several genomic libraries in a lambda phage vector (Lambda Fix II; Stratagene, La Jolla, Calif.) had been constructed within the present inventors' laboratories.



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